|
KMID : 0624620090420070462
|
|
BMB Reports
2009 Volume.42 No. 7 p.462 ~ p.466
|
|
|
Effects of protein concentration and detergent on endotoxin reduction by ultrafiltration
|
|
Jang Hyun
Kim Hyo-Seung
Moon Seung-Cheol
Lee Young-Rae
Yu Kang-Yeoul
Kim Jong-Suk
Lee Byeong-Kil
Youn Hyun-Zo
Jeong Young-Ju
Kim Byeong-Soo
Lee Sung-Ho
|
|
|
Abstract
|
|
|
|
Lipopolysaccharide (LPS), found in the outer membrane of Gram negative bacteria, only exerts its toxic effects when in free form. LPS has three major parts, lipid A, the toxic component, along with a core polysaccharide and O-specific polysaccharide. LPS monomers are known to have molecular masses between 10 to 30 kDa. Under physiological conditions, LPS exists in equilibrium between monomer and vesicle forms. LPS removal by 100 kDa ultrafiltration was more efficient (99.6% of LPS removed) with a low concentration of protein (2.0 mg/ml) compared to a high concentration (20.1 mg/ml). In the presence of different detergents (0.5% Tween 20, 1.0% taurodeoxycholate and 1.0% Triton X-100), LPS removal was more efficient at low protein concentrations (2.0 mg/ml) compared to high protein concentrations (20.1 mg/ml).
|
|
|
KEYWORD
|
|
|
Chromatography, Detergent, Endotoxin, Lipopolysaccharide (LPS), Ultrafiltration
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|